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Hydatidosis Diagnosis With Novel ELISA Kits

Hydatidosis Diagnosis With Novel ELISA Kits

Nisreen Waleed Mustafa; Wafaa Sadoon Shani

Lap Lambert Academic Publishing
2024
pokkari
This study included five antigens, each examined with three immunoglobulins (total IgG, IgG1, and IgG4) using chequerboard titration ELISA to determine the optimal antigen concentration and serum dilutions for preparing diagnostic kits. The indirect ELISA method was then used to evaluate the diagnostic performance values (DPV) (sensitivity, specificity, diagnostic efficiency, positive predictive value, and negative predictive value) based on the cutoff value of each antigen with total IgG and its subclasses (IgG1 and IgG4). Antigen B (AgB) was purified from hydatid fluid and electrophoretically treated to isolate its bands (8 kDa, 16 kDa, and 24 kDa AgB), which were used as antigens, in addition to an antigen purified from protoscoleces. Among the 15 prepared kits, those containing the 8 kDa subunit of AgB and IgG4 showed the best performance. Results from the ROC curve indicated that the 8 kDa subunit had the highest diagnostic accuracy when tested with IgG4 and IgG1. Additionally, this study included the immunization of rabbits with AgB, and the results indicated that AgB can modulate the immune response toward a Th2 bias.
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Hydatidose-Diagnose mit neuartigen ELISA-Kits

Hydatidose-Diagnose mit neuartigen ELISA-Kits

Nisreen Waleed Mustafa; Wafaa Sadoon Shani

Verlag Unser Wissen
2025
pokkari
Diese Studie umfasste f nf Antigene, die jeweils mit drei Immunglobulinen (Gesamt-IgG, IgG1 und IgG4) unter Verwendung von Schachbrett-Titration-ELISA untersucht wurden, um die optimale Antigenkonzentration und Serumverd nnung f r die Herstellung von Diagnosekits zu bestimmen. Mit der indirekten ELISA-Methode wurden dann die diagnostischen Leistungswerte (DPV) (Sensitivit t, Spezifit t, diagnostische Effizienz, positiver pr diktiver Wert und negativer pr diktiver Wert) auf der Grundlage des Cutoff-Werts jedes Antigens mit Gesamt-IgG und seinen Unterklassen (IgG1 und IgG4) bewertet. Antigen B (AgB) wurde aus Hydatidenfl ssigkeit gereinigt und elektrophoretisch behandelt, um seine Banden (8 kDa, 16 kDa und 24 kDa AgB) zu isolieren, die als Antigene verwendet wurden, zus tzlich zu einem aus Protoskolekten gereinigten Antigen. Von den 15 vorbereiteten Kits zeigten diejenigen, die die 8 kDa-Untereinheit von AgB und IgG4 enthielten, die beste Leistung. Die Ergebnisse der ROC-Kurve zeigten, dass die 8 kDa-Untereinheit die h chste diagnostische Genauigkeit aufwies, wenn sie mit IgG4 und IgG1 getestet wurde. Dar ber hinaus umfasste diese Studie die Immunisierung von Kaninchen mit AgB, und die Ergebnisse deuteten darauf hin, dass AgB die Immunantwort in Richtung einer Th2-Neigung modulieren kann.
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Diagnostic de l'hydatidose à l'aide de nouveaux kits ELISA

Diagnostic de l'hydatidose à l'aide de nouveaux kits ELISA

Nisreen Waleed Mustafa; Wafaa Sadoon Shani

Editions Notre Savoir
2025
pokkari
Cette tude a port sur cinq antig nes, chacun examin avec trois immunoglobulines (IgG totale, IgG1 et IgG4) l'aide de la m thode ELISA de titrage en damier afin de d terminer la concentration optimale de l'antig ne et les dilutions de s rum pour la pr paration des kits de diagnostic. La m thode ELISA indirecte a ensuite t utilis e pour valuer les valeurs de performance diagnostique (sensibilit , sp cificit , efficacit diagnostique, valeur pr dictive positive et valeur pr dictive n gative) sur la base de la valeur seuil de chaque antig ne avec l'IgG totale et ses sous-classes (IgG1 et IgG4). L'antig ne B (AgB) a t purifi partir du liquide hydatique et trait par lectrophor se pour isoler ses bandes (AgB 8 kDa, 16 kDa et 24 kDa), qui ont t utilis es comme antig nes, en plus d'un antig ne purifi partir de protoscoleces. Parmi les 15 kits pr par s, ceux contenant la sous-unit 8 kDa de l'AgB et l'IgG4 ont montr les meilleures performances. Les r sultats de la courbe ROC ont indiqu que la sous-unit 8 kDa avait la plus grande pr cision diagnostique lorsqu'elle tait test e avec les IgG4 et les IgG1. En outre, cette tude comprenait l'immunisation de lapins avec l'AgB, et les r sultats ont indiqu que l'AgB peut moduler la r ponse immunitaire vers un biais Th2.
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Diagnosi dell'idatidosi con nuovi kit ELISA

Diagnosi dell'idatidosi con nuovi kit ELISA

Nisreen Waleed Mustafa; Wafaa Sadoon Shani

Edizioni Sapienza
2025
pokkari
Questo studio ha incluso cinque antigeni, ciascuno esaminato con tre immunoglobuline (IgG totali, IgG1 e IgG4) utilizzando il metodo ELISA a titolazione a scacchiera per determinare la concentrazione ottimale dell'antigene e le diluizioni del siero per la preparazione dei kit diagnostici. Il metodo ELISA indiretto stato poi utilizzato per valutare i valori di performance diagnostica (DPV) (sensibilit , specificit , efficienza diagnostica, valore predittivo positivo e valore predittivo negativo) in base al valore di cutoff di ciascun antigene con le IgG totali e le sue sottoclassi (IgG1 e IgG4). L'antigene B (AgB) stato purificato dal liquido idatideo e trattato elettroforeticamente per isolare le sue bande (8 kDa, 16 kDa e 24 kDa AgB), che sono state utilizzate come antigeni, oltre a un antigene purificato dalle protoscolecole. Tra i 15 kit preparati, quelli contenenti la subunit 8 kDa di AgB e le IgG4 hanno mostrato le migliori prestazioni. I risultati della curva ROC hanno indicato che la subunit 8 kDa ha avuto la massima accuratezza diagnostica quando stata testata con IgG4 e IgG1. Inoltre, questo studio ha incluso l'immunizzazione di conigli con AgB e i risultati hanno indicato che AgB pu modulare la risposta immunitaria verso un orientamento Th2.
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Diagnostyka hydatidozy za pomoca nowych zestawów ELISA

Diagnostyka hydatidozy za pomoca nowych zestawów ELISA

Nisreen Waleed Mustafa; Wafaa Sadoon Shani

Wydawnictwo Nasza Wiedza
2025
pokkari
Badanie to obejmowalo pięc antygen w, z kt rych każdy byl badany z trzema immunoglobulinami (calkowita IgG, IgG1 i IgG4) przy użyciu testu ELISA z miareczkowaniem szachownicy w celu określenia optymalnego stężenia antygenu i rozcieńczeń surowicy do przygotowania zestaw w diagnostycznych. Pośrednia metoda ELISA zostala następnie wykorzystana do oceny wartości wydajności diagnostycznej (DPV) (czulośc, swoistośc, skutecznośc diagnostyczna, dodatnia wartośc predykcyjna i ujemna wartośc predykcyjna) w oparciu o wartośc odcięcia każdego antygenu z calkowitą IgG i jej podklasami (IgG1 i IgG4). Antygen B (AgB) oczyszczono z plynu hydatidowego i poddano obr bce elektroforetycznej w celu wyizolowania jego pasm (8 kDa, 16 kDa i 24 kDa AgB), kt re wykorzystano jako antygeny, opr cz antygenu oczyszczonego z protoskolec w. Spośr d 15 przygotowanych zestaw w, te zawierające podjednostkę 8 kDa AgB i IgG4 wykazaly najlepszą wydajnośc. Wyniki z krzywej ROC wskazaly, że podjednostka 8 kDa miala najwyższą dokladnośc diagnostyczną, gdy byla testowana z IgG4 i IgG1. Ponadto badanie to obejmowalo immunizację kr lik w AgB, a wyniki wskazywaly, że AgB może modulowac odpowiedź immunologiczną w kierunku nastawienia Th2.
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Diagnóstico da hidatidose com novos kits ELISA

Diagnóstico da hidatidose com novos kits ELISA

Nisreen Waleed Mustafa; Wafaa Sadoon Shani

Edicoes Nosso Conhecimento
2025
pokkari
Este estudo incluiu cinco antig nios, cada um examinado com tr s imunoglobulinas (IgG total, IgG1 e IgG4) utilizando o ELISA de titula o em tabuleiro de xadrez para determinar a concentra o ideal de antig nio e as dilui es de soro para a prepara o de kits de diagn stico. O m todo ELISA indireto foi ent o utilizado para avaliar os valores de desempenho de diagn stico (DPV) (sensibilidade, especificidade, efici ncia de diagn stico, valor preditivo positivo e valor preditivo negativo) com base no valor de corte de cada antig nio com IgG total e as suas subclasses (IgG1 e IgG4). O antig nio B (AgB) foi purificado a partir do l quido hid tico e tratado electroforeticamente para isolar as suas bandas (AgB de 8 kDa, 16 kDa e 24 kDa), que foram utilizadas como antig nios, para al m de um antig nio purificado a partir de protocolecas. Entre os 15 kits preparados, os que continham a subunidade de 8 kDa do AgB e IgG4 apresentaram o melhor desempenho. Os resultados da curva ROC indicaram que a subunidade de 8 kDa teve a maior precis o de diagn stico quando testada com IgG4 e IgG1. Al m disso, este estudo incluiu a imuniza o de coelhos com AgB, e os resultados indicaram que o AgB pode modular a resposta imunit ria no sentido de uma tend ncia Th2.
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Learning by Design

Learning by Design

A. B. (Rami) Shani; Peter Docherty

JOHN WILEY AND SONS LTD
2003
nidottu
This book advances a design-based approach for the investigation and creation of sustainable organizations. The learning-by-design framework is utilized to examine learning in six successful companies in different industries and national settings and provides a roadmap for improving systematic learning in organizations. Investigates learning-by-design in successful companies. Focuses on the choices organizations make about the design of learning mechanisms. Built around six detailed case studies taken from different industries and national settings. Provides a framework for improving the conditions for systematic and sustainable learning in organizations. Offers a clear process model for action and change.
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Conducting Action Research for Business and Management Students

Conducting Action Research for Business and Management Students

David Coghlan; Abraham B. Shani

SAGE Publications Ltd
2018
sidottu
In Conducting Action Research, Coghlan and Shani explain how action research differs from more detached research methods and provides expert guidance on how to engage effectively with it, helping the reader to complete both a successful research project and produce findings that are useful in an organizational context. Ideal for Business and Management students reading for a Master’s degree, each book in the series may also serve as reference books for doctoral students and faculty members interested in the method. Part of SAGE's Mastering Business Research Methods, conceived and edited by Bill Lee, Mark N. K. Saunders and Vadake K. Narayanan and designed to support researchers by providing in-depth and practical guidance on using a chosen method of data collection or analysis.
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Conducting Action Research for Business and Management Students

Conducting Action Research for Business and Management Students

David Coghlan; Abraham B. Shani

SAGE Publications Ltd
2018
nidottu
In Conducting Action Research, Coghlan and Shani explain how action research differs from more detached research methods and provides expert guidance on how to engage effectively with it, helping the reader to complete both a successful research project and produce findings that are useful in an organizational context. Ideal for Business and Management students reading for a Master’s degree, each book in the series may also serve as reference books for doctoral students and faculty members interested in the method. Part of SAGE's Mastering Business Research Methods, conceived and edited by Bill Lee, Mark N. K. Saunders and Vadake K. Narayanan and designed to support researchers by providing in-depth and practical guidance on using a chosen method of data collection or analysis.
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Storymaking and Organizational Transformation

Storymaking and Organizational Transformation

Tommaso Buganza; Paola Bellis; Silvia Magnanini; Joseph Press; Abraham (Rami) B. Shani; Daniel Trabucchi; Roberto Verganti; Federico Paolo Zasa

TAYLOR FRANCIS LTD
2022
sidottu
In a world undergoing continuous change, organizations find themselves facing the challenge of how to keep innovating to stay competitive. Inside any organization, people are the cornerstone on which innovation rests and builds, yet it is ever more difficult to engage everyone in designing their organization. This book explores and discusses how employees can be engaged digitally to assist innovation initiatives and lead to organizational transformation.Storymaking and Organizational Transformation is based on the research activities of the platform IDeaLs during the year 2020 and provides a perspective on how employees can be helped to understand and even contribute to organizational innovation spontaneously. The book contributes to advancing understanding of engagement from two main perspectives: first, the authors introduce an approach based on storymaking; second, six cases are studied in depth and the application of the digital storymaking approach is explained. The authors introduce new ways of organizing in a context of ongoing change, as they bring forth the idea that engagement is a continuous practice of designing meaningful narratives which connect people and evolve along with them.The book will appeal to both academics and practitioners across management fields. Scholars of innovation management and organization sciences will benefit from the extensive review of organizational transformation and innovation from a sensemaking perspective, whilst the practical, case studies provide a valuable resource for practitioners looking to effect change and manage transformation.
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Storymaking and Organizational Transformation

Storymaking and Organizational Transformation

Tommaso Buganza; Paola Bellis; Silvia Magnanini; Joseph Press; Abraham (Rami) B. Shani; Daniel Trabucchi; Roberto Verganti; Federico Paolo Zasa

TAYLOR FRANCIS LTD
2024
nidottu
In a world undergoing continuous change, organizations find themselves facing the challenge of how to keep innovating to stay competitive. Inside any organization, people are the cornerstone on which innovation rests and builds, yet it is ever more difficult to engage everyone in designing their organization. This book explores and discusses how employees can be engaged digitally to assist innovation initiatives and lead to organizational transformation.Storymaking and Organizational Transformation is based on the research activities of the platform IDeaLs during the year 2020 and provides a perspective on how employees can be helped to understand and even contribute to organizational innovation spontaneously. The book contributes to advancing understanding of engagement from two main perspectives: first, the authors introduce an approach based on storymaking; second, six cases are studied in depth and the application of the digital storymaking approach is explained. The authors introduce new ways of organizing in a context of ongoing change, as they bring forth the idea that engagement is a continuous practice of designing meaningful narratives which connect people and evolve along with them.The book will appeal to both academics and practitioners across management fields. Scholars of innovation management and organization sciences will benefit from the extensive review of organizational transformation and innovation from a sensemaking perspective, whilst the practical, case studies provide a valuable resource for practitioners looking to effect change and manage transformation.
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Shane Comes Home

Shane Comes Home

Rinker Buck

William Morrow Company
2006
nidottu
An award-winning journalist and author of Flight of Passage provides an affecting portrait of Marine Lieutenant Shane Childers, the first American soldier to be killed during the conflict in Iraq, reflecting on the meaning of his death in terms of his family, his fellow soldiers, and the American nation. Reprint.
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Shari'a and Muslim Minorities

Shari'a and Muslim Minorities

Uriya Shavit

Oxford University Press
2015
sidottu
Based on a comparative analysis of several hundred religio-juristic treatises and fatwas (religious decisions), Shari'a and Muslim Minorities: The Wasati and Salafi Approaches to Fiqh al-Aqalliyyat al-Muslima offers the most systematic and comprehensive study to date of fiqh al aqalliyyat al-Muslima - the field in Islamic jurisprudence that treats issues that are unique to Muslims living in majority non-Muslim societies. The book argues that two main contesting approaches to fiqh al-aqalliyyat al-Muslima, the wasati and the salafi, have developed, in part dialectically. While both envision a future Islamizing of the West as a main justification for Muslim residence in the West, the wasati approach is pragmatic, facilitating, and integration-minded, whereas the salafi calls for strict application of religious norms and for introversion. The volume examines diverse and highly-debated juristic issues, including the permissibility of naturalizing in non-Muslim states, participating in their electoral systems and serving in their militaries and police forces; the permissibility of taking mortgages and student loans; the permissibility of congratulating Christians on Christmas or receiving Christmas bonuses; and the permissibility of working in professions that involve breaching of religio-legal prohibitions (e.g. serving pork). Discussions highlight the diversity within contemporary Islamic jurisprudence and introduce new nuances to highly-charged concepts such as proselytizing, integration, and multiculturalism.
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Shari'a in the West

Shari'a in the West

Oxford University Press
2010
sidottu
In February 2008, the Archbishop of Canterbury, Dr Rowan Williams, delivered a public lecture in which he stated that it "seem[ed] unavoidable" that certain aspects of Islamic law (Shari'a) would be recognized and incorporated into British law. The comments provoked outrage from sections of the public who viewed any recognition of Shari'a law in Britain with alarm. In July 2008 Lord Phillips, Lord Chief Justice of England and Wales, weighed into the fray. He praised the Archbishop's speech and gave qualified support for Shari'a principles to govern certain family and civil disputes. Responding to the polarised debate that followed these lectures, this is a collection of short essays written by distinguished and prominent scholars addressing the question of the accommodation of Shari'a within the legal systems of the liberal-democratic West. The matters raised in the two 2008 lectures provide a springboard for lively discussion, criticism and debate on both the specific question of religious/cultural accommodation by the law and the wider issues of multiculturalism, equality before the law and the desirability of parallel jurisdictions for particular faith communities. Leading scholars from a range of countries and academic disciplines, and representing different political viewpoints and faith traditions explore the complex issues surrounding the legal recognition of religious faith in a multicultural society. The volume aims to stimulate further thought on a complex issue, and to open up new pathways for policymakers and civil society institutions grappling with the relationship between Shari'a and Western legal systems.
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Shari'a and Social Engineering

Shari'a and Social Engineering

R. Michael Feener

Oxford University Press
2013
sidottu
This book seeks to open new lines of discussion about how Islamic law is viewed as a potential tool for programs of social transformation in contemporary Muslim society. It does this through a critical examination of the workings of the state shari'a system as it was designed and implemented at the turn of the twenty-first century in Aceh, Indonesia. While the empirical details of these discussions are unique, this particular case presents a remarkable site for investigating the broader issue of the impact of instrumentalist, future-oriented visions of Islamic law on modern Muslim calls for the state implementation of Islamic law. In post-tsunami/post-conflict Aceh, the idea of shari'a as an exercise in social engineering was amplified through resonance with an increasingly pervasive rhetoric of 'total reconstruction'. Based upon extensive fieldwork as well as critical readings of a wide range of archival materials, official documents, and local publications this work focuses on the institutions and actors involved with this contemporary project for the state implementation of Islamic law. The individual chapters are structured to deal with the major components of this system to critically examine how these institutions have taken shape and how they work. It also shows how the overall system was informed not only by aspects of late twentieth-century da'wa discourses of Islamic reform, but also modern trends in sociological jurisprudence and the impact of global models of disaster relief, reconstruction, and development. All of these streams of influence have contributed significantly to shaping the ways in which the architects and agents of the state shari'a system have attempted to use Islamic legislation and legal institutions as tools to steer society in particular desired directions. This is an open access title available under the terms of a CC BY-NC-ND 4.0 International licence. It is free to read at Oxford Scholarship Online and offered as a free PDF download from OUP and selected open access locations.
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Shari'a Scripts

Shari'a Scripts

Brinkley Messick

Columbia University Press
2018
sidottu
A case study in the textual architecture of the venerable legal and ethical tradition at the center of the Islamic experience, Shari'a Scripts is a work of historical anthropology focused on Yemen in the early twentieth century. While colonial regimes, late Ottoman reformers, and early nationalists wrought decisive changes to the legal status of the shari'a, significantly narrowing its sphere of relevance, the Zaydi school of jurisprudence, rooted in highland Yemen for a millennium, still held sway. Brinkley Messick uses the richly varied writings of the Yemeni past to offer a uniquely comprehensive view of the shari'a as a localized and lived phenomenon. Shari'a Scripts reads a wide spectrum of sources in search of a new historical-anthropological perspective on Islamic textual relations. Messick analyzes the shari'a as a local system of texts, distinguishing between theoretical or doctrinal juridical texts (or the "library") and those produced by the shari'a courts and notarial writers (termed the "archive"). Attending to textual form, he closely examines representative books of madrasa instruction; formal opinion-giving by muftis and imams; the structure of court judgments; and the drafting of contracts. Messick's intensive readings of texts are supplemented by retrospective ethnography and oral history based on extensive field research. Shari'a Scripts also ventures a major methodological contribution by confronting anthropology's longstanding reliance upon the observational and the colloquial, seeking to develop tools for the anthropologist as reader.
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Shari'a Scripts

Shari'a Scripts

Brinkley Messick

Columbia University Press
2022
pokkari
A case study in the textual architecture of the venerable legal and ethical tradition at the center of the Islamic experience, Shari?a Scripts is a work of historical anthropology focused on Yemen in the early twentieth century. There—while colonial regimes, late Ottoman reformers, and early nationalists wrought decisive changes to the legal status of the shari?a, significantly narrowing its sphere of relevance—the Zaydi school of jurisprudence, rooted in highland Yemen for a millennium, still held sway.Brinkley Messick uses the richly varied writings of the Yemeni past to offer a uniquely comprehensive view of the shari?a as a localized and lived phenomenon. Shari?a Scripts reads a wide spectrum of sources in search of a new historical-anthropological perspective on Islamic textual relations. Messick analyzes the shari?a as a local system of texts, distinguishing between theoretical or doctrinal juridical texts (or the “library”) and those produced by the shari?a courts and notarial writers (termed the “archive”). Attending to textual form, he closely examines representative books of madrasa instruction; formal opinion-giving by muftis and imams; the structure of court judgments; and the drafting of contracts. Messick’s intensive readings of texts are supplemented by retrospective ethnography and oral history based on extensive field research. Further, the book ventures a major methodological contribution by confronting anthropology’s longstanding reliance upon the observational and the colloquial. Presenting a new understanding of Islamic legal history, Shari?a Scripts is a groundbreaking examination of the interpretative range and historical insights offered by the anthropologist as reader.
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Shari'a Law and Modern Muslim Ethics

Shari'a Law and Modern Muslim Ethics

Indiana University Press
2016
sidottu
Many Muslim societies are in the throes of tumultuous political transitions, and common to all has been heightened debate over the place of sharia law in modern politics and ethical life. Bringing together leading scholars of Islamic politics, ethics, and law, this book examines the varied meanings and uses of Islamic law, so as to assess the prospects for democratic, plural, and gender-equitable Islamic ethics today. These essays show that, contrary to the claims of some radicals, Muslim understandings of Islamic law and ethics have always been varied and emerge, not from unchanging texts but from real and active engagement with Islamic traditions and everyday life. The ethical debates that rage in contemporary Muslim societies reveal much about the prospects for democratic societies and a pluralist Islamic ethics in the future. They also suggest that despite the tragic violence wrought in recent years by Boko Haram and the Islamic State in Iraq, we may yet see an age of ethical renewal across the Muslim world.
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Shari'a Law and Modern Muslim Ethics

Shari'a Law and Modern Muslim Ethics

Indiana University Press
2016
pokkari
Many Muslim societies are in the throes of tumultuous political transitions, and common to all has been heightened debate over the place of sharia law in modern politics and ethical life. Bringing together leading scholars of Islamic politics, ethics, and law, this book examines the varied meanings and uses of Islamic law, so as to assess the prospects for democratic, plural, and gender-equitable Islamic ethics today. These essays show that, contrary to the claims of some radicals, Muslim understandings of Islamic law and ethics have always been varied and emerge, not from unchanging texts but from real and active engagement with Islamic traditions and everyday life. The ethical debates that rage in contemporary Muslim societies reveal much about the prospects for democratic societies and a pluralist Islamic ethics in the future. They also suggest that despite the tragic violence wrought in recent years by Boko Haram and the Islamic State in Iraq, we may yet see an age of ethical renewal across the Muslim world.
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