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Analyse von Malariamitteln in menschlichem Plasma mittels LC-MS/MS

Analyse von Malariamitteln in menschlichem Plasma mittels LC-MS/MS

Jaivik Shah; Priyanka Shah; Shanti Swaroop Yadav

Verlag Unser Wissen
2023
pokkari
Die Methode stellt ein effizientes Extraktionsverfahren auf der Grundlage der Fl ssig-Fl ssig-Extraktion (LLE) dar, das eine hohe und konsistente Wiederfindung f r beide Analyten erm glicht. Der Matrixeffekt wurde gr ndlich bewertet, indem die IS-normalisierten Matrixfaktoren auch durch Infusion des Analyten nach der S ule gesch tzt wurden. Die entwickelte LC-MS/MS-Methode f r die Quantifizierung von ART, DHA, LF und DLF in menschlichem Plasma wurde gem den Richtlinien der US FDA vollst ndig validiert. Die vorgeschlagene Methode ist f r beide Analyten empfindlich im Vergleich zu allen anderen Methoden, die entweder als Einzelanalyt oder in Kombination in verschiedenen biologischen Matrices vorgestellt wurden. Die Methode bietet im Vergleich zu den bisher vorgestellten Methoden erhebliche Vorteile in Bezug auf den geringeren Probenbedarf f r die Analyse, die Einfachheit des Extraktionsverfahrens und die Gesamtanalysezeit. Die Abwesenheit von Matrixinterferenzen wird durch die Nachs uleninfusion und durch die Pr zisionswerte (% CV) f r die berechneten Steigungen der Kalibrierkurven effektiv nachgewiesen. Die validierte Methode zeigte akzeptable Daten f r alle Validierungsparameter mit angemessener Empfindlichkeit und Selektivit t f r ihre gleichzeitige Quantifizierung in einem klinischen Umfeld.
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Analyse des médicaments antipaludiques dans le plasma humain par LC-MS/MS

Analyse des médicaments antipaludiques dans le plasma humain par LC-MS/MS

Jaivik Shah; Priyanka Shah; Shanti Swaroop Yadav

Editions Notre Savoir
2023
pokkari
La m thode pr sente une proc dure d'extraction efficace bas e sur l'extraction liquide-liquide (ELL) qui permet une r cup ration lev e et constante des deux analytes. L'effet de matrice a t valu de mani re approfondie en estimant les facteurs de matrice normalis s par l'IS, ainsi que par la perfusion post-colonne de l'analyte. La m thode LC-MS/MS d velopp e pour la quantification de l'ART, du DHA, du LF et du DLF dans le plasma humain a t enti rement valid e conform ment aux directives de la FDA am ricaine. La m thode propos e est sensible pour les deux analytes par rapport toutes les autres m thodes pr sent es soit en tant qu'analyte unique soit en combinaison dans diff rentes matrices biologiques. La m thode offre des avantages significatifs par rapport celles pr c demment rapport es, en termes de faible besoin en chantillons pour l'analyse, de simplicit de la proc dure d'extraction et de temps d'analyse global. L'absence d'interf rence de la matrice est effectivement d montr e par la perfusion post-colonne et par les valeurs de pr cision (% CV) pour les pentes calcul es des courbes d' talonnage. La m thode valid e a montr des donn es acceptables pour tous les param tres de validation, avec une sensibilit et une s lectivit ad quates pour leur quantification simultan e dans un contexte clinique.
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Analisi dei farmaci antimalarici nel plasma umano mediante LC-MS/MS

Analisi dei farmaci antimalarici nel plasma umano mediante LC-MS/MS

Jaivik Shah; Priyanka Shah; Shanti Swaroop Yadav

Edizioni Sapienza
2023
pokkari
Il metodo presenta una procedura di estrazione efficiente basata sull'estrazione liquido-liquido (LLE) che fornisce un recupero elevato e costante per entrambi gli analiti. L'effetto matrice stato accuratamente valutato stimando i fattori di matrice normalizzati IS anche attraverso l'infusione dell'analita dopo la colonna. Il metodo LC-MS/MS sviluppato per la quantificazione di ART, DHA, LF e DLF nel plasma umano stato completamente validato secondo le linee guida della FDA statunitense. Il metodo proposto sensibile per entrambi gli analiti rispetto a tutti gli altri metodi presentati sia come singolo analita che in combinazione in diverse matrici biologiche. Il metodo offre vantaggi significativi rispetto a quelli precedentemente riportati, in termini di minore necessit di campioni per l'analisi, semplicit della procedura di estrazione e tempo complessivo di analisi. L'assenza di interferenze della matrice efficacemente dimostrata dall'infusione postcolonna e dai valori di precisione (% CV) per le pendenze calcolate delle curve di calibrazione. Il metodo validato ha mostrato dati accettabili per tutti i parametri di validazione, con sensibilit e selettivit adeguate per la loro quantificazione simultanea in ambito clinico.
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Análise de medicamentos antimaláricos no plasma humano por LC-MS/MS

Análise de medicamentos antimaláricos no plasma humano por LC-MS/MS

Jaivik Shah; Priyanka Shah; Shanti Swaroop Yadav

Edicoes Nosso Conhecimento
2023
pokkari
O m todo apresenta um procedimento de extrac o eficiente baseado na extrac o l quido-l quido (LLE) que proporciona uma recupera o elevada e consistente tanto para os analitos como para os analitos. O efeito da matriz foi cuidadosamente avaliado atrav s da estimativa dos factores da matriz normalizada IS tamb m atrav s da infus o de analitos p s-coluna. O m todo LC-MS/MS desenvolvido para a quantifica o de ART, DHA, LF e DLF em plasma humano foi totalmente validado de acordo com as directrizes da FDA dos EUA. O m todo proposto sens vel para ambos os analitos em compara o com todos os outros m todos apresentados quer como um nico analito quer em combina o em diferentes matrizes biol gicas. O m todo oferece vantagens significativas sobre os anteriormente relatados, em termos de menor exig ncia de amostras para an lise, simplicidade do procedimento de extrac o e tempo global de an lise. A aus ncia de interfer ncia da matriz efectivamente demonstrada pela infus o p s-coluna e pelos valores de precis o (% CV) para as inclina es calculadas das curvas de calibra o. O m todo validado mostrou dados aceit veis para todos os par metros de valida o, com sensibilidade e selectividade adequadas para a sua quantifica o simult nea num cen rio cl nico.
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Navigating Complexities Faced by Pharmacists in India

Navigating Complexities Faced by Pharmacists in India

Manjit Rajput; Akbar Firoz; Shanti Mishra

Lap Lambert Academic Publishing
2023
pokkari
Navigating Complexities: A Thorough Examination of Challenges Faced by Pharmacists in India is a comprehensive exploration of the multifaceted challenges encountered by pharmacists in the dynamic landscape of India. Authored by industry experts and thought leaders, this book provides an in-depth analysis of the hurdles, opportunities, and evolving trends that define the professional journey of pharmacists in the country. A forward-looking section providing recommendations and directions for pharmacists, educators, policymakers, and stakeholders to shape a progressive and patient-centered future for pharmacy practice in India.
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Myofunctional Appliances in Children

Myofunctional Appliances in Children

Treesa Mary Joseph; Zareena M a; Shanthala B M

Lap Lambert Academic Publishing
2024
pokkari
This book offers a comprehensive guide to the role of myofunctional appliances in pediatric dentistry, exploring both the science and practical applications of early intervention in children's dental health. It delves into commonly used myofunctional appliances during the mixed dentition period, providing detailed discussions on their use.Emphasizes the importance of addressing functional and structural issues from a young age. The book focuses on the use of myofunctional appliances in critical stages of developmental in children. Through in-depth insights, it examines how myofunctional appliances can guide facial growth and promote proper jaw alignment-laying the foundation for a lifetime of healthy smiles.The adage "catch them young, watch them grow" reflects the core philosophy of this approach: by identifying and treating dental issues early, we can support optimal growth and development. Offering valuable insights and practical applications.
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Broadcast Gadget for cars using Sensors & GPS Module

Broadcast Gadget for cars using Sensors & GPS Module

Y Sudha; G Ravi Kishore; S Santhi Priya

Lap Lambert Academic Publishing
2024
pokkari
The main purpose of this wireless black box is to develop a vehicle black box system that can be installed into any vehicle all over the world. This paradigm is often designed with minimum range of circuits. Wireless black box is basically a device that will indicate all the parameters of a vehicle crash and will also store and display its parameters at every three seconds such as date, time, temperature, location, vibration, alcohol limit etc. At the time of accident, the message will be sent from the system built inside the car to the registered mobile numbers such as emergency numbers of police stations, hospitals, family members, owner etc. We have used various types of sensors like temperature sensor (DTH11), which is used to measure temperature and humidity. Vibration sensor measures vibrations felt by the car during accident. Alcohol sensors are located on the steering wheel which will indicate whether the driver is drunk. Gyroscopic sensor is used to indicate tilt during the accident. All the parameters sensed by the sensors will send the signal to Arduino Uno. GSM module, SD card module, GPS module are some of the devices used which helps in accomplishing the output.
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Appareils Myofonctionnels Chez l'Enfant

Appareils Myofonctionnels Chez l'Enfant

Treesa Mary Joseph; Zareena M a; Shanthala B M

Editions Notre Savoir
2024
pokkari
Ce livre offre un guide complet sur le r le des appareils myofonctionnels en dentisterie p diatrique, explorant la fois la science et les applications pratiques de l'intervention pr coce dans la sant dentaire des enfants. Il se penche sur les appareils myofonctionnels couramment utilis s pendant la p riode de dentition mixte, en fournissant des discussions d taill es sur leur utilisation.Il souligne l'importance de traiter les probl mes fonctionnels et structurels d s le plus jeune ge. Le livre se concentre sur l'utilisation des appareils myofonctionnels aux stades critiques du d veloppement de l'enfant. Gr ce des observations approfondies, il examine comment les appareils myofonctionnels peuvent guider la croissance faciale et promouvoir un alignement correct des m choires, jetant ainsi les bases d'un sourire sain tout au long de la vie.L'adage "attrapez-les jeunes, regardez-les grandir" refl te la philosophie fondamentale de cette approche: en identifiant et en traitant les probl mes dentaires un stade pr coce, nous pouvons favoriser une croissance et un d veloppement optimaux. Offrant des informations pr cieuses et des applications pratiques.
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