Kirjahaku
Etsi kirjoja tekijän nimen, kirjan nimen tai ISBN:n perusteella.
1000 tulosta hakusanalla Elisa Leonelli
7he a1m 0f EL15A: 7he0ry and Pract1ce t0 1ntr0duce the expe- menter t0 the techn0109y kn0wn the En2yme L1nked 1mmun050r6ent A55ay (EL15A). 7he 600k d1rected at w0rker5 w1th vary1n9 de9ree5 0f exper1ence, 6ut f0r th05e w1th0ut any prev10u5 c0ntact w1th the a55ay. 1mmun0a55ay5 f0rm the 6ack60ne 0f u5ed te5t5 the 5tudy 0f 1nf- d15ea5e5 t10u5 and end0cr1n0109y, and hence can 6e u5ed t01d- a9ent5 cau5at1ve t1fy 0f the extent and nature (ep1dem10109y) 0f d15ea5e, and mea5ure h0rm0ne5 at EL15A5 pr0v1de h19h1y and prec15e meth0d5 f0r the mat10n 0f parameter5, w1th the added advanta9e that they can hand1e 1ar9e num6er5 0f 5amp1e5 that may then 6e ana1y2ed rap1d1y. 7he5e a55ay5 are ver5at11e, hav1n9 6een app11ed t0 a w1de ran9e 0f e.9., v1ru5e5, 6acter1a, fun91, and pr0t020an and meta- 20an 51nce para51te5. 1971, when the effect1ve en2yme-1a6e1ed a55ay wa5 de5cr16ed (1), th0u5and5 0f have 6een pu6115hed de- w1th the 4uant1f1cat10n 0f ant19en5 and ant160d1e5 f0r re5earch and app11ed purp05e5. EL15A and re1ated a55ay5 1nv01v1n9 u5e 0f en2yme5 t0 06ta1n c010r1metr1c re5u1t5 have n0w rep1aced rad101mmun0a55ay (R1A) f0r m05t d1a9n05t1c purp05e5, 51nce the f0rmer 0ffer5 a p0tent1a1 w1th 1ncrea5ed ver5at111ty t0 a w1der 9r0up 0f Examp1e5 0f 5pec1f1c area5 t0 wh1ch EL15A5 have 6een app11ed re5earch and are: Detect10n and 0f wh01e 0r part5 0f a9ent5, e.9., typ1n9; 2. 0f a9ent5, e.9., 5u6typ1n9; d15ea5e D15cr1m1nat10n d15ea5e 1dent1f1cat10n 1. d1a9n0515 1n 5c1ent15t5.
ELISA
SPRINGER-VERLAG NEW YORK INC.
2023
sidottu
This volume provides an understanding of how an immunoassay works, detailing the strengths, weaknesses, pitfalls. Chapters guide readers on how and when to appropriately utilize this powerful tool, examples of where the ELISA or similar immunoassay formats are currently being used, and newer techniques that may have a significant impact on future applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, ELISA: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
ELISA
SPRINGER-VERLAG NEW YORK INC.
2024
nidottu
This volume provides an understanding of how an immunoassay works, detailing the strengths, weaknesses, pitfalls. Chapters guide readers on how and when to appropriately utilize this powerful tool, examples of where the ELISA or similar immunoassay formats are currently being used, and newer techniques that may have a significant impact on future applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, ELISA: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
lisa a non seulement un pass douloureux, mais elle entretient galement une relation toxique avec un homme alcoolique et violent. Son amie Effy d cide d' tablir un plan qui, selon elle, la sauvera de cet enfer. Elle entra ne alors ses deux autres acolytes, Lili et Sarah, dans sa machination machiav lique, malgr les r ticences raisonn es de leur confidente, Maddie.
ELISA
Humana Press Inc.
2015
sidottu
This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
ELISA
Humana Press Inc.
2016
nidottu
This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Suite d'une trange affaire. Une adolescente a disparu sur le bref trajet entre la maison de son amie et la sienne. Kochka, devenu un v ritable d tective, est charg de l'enqu te qui s'annonce tortueuse sur fond d'espionnage industriel.
Elisa is about a young woman from Tanzania who is very adventurous and loves to explore. Her father wants her to marry a man from her village, but she wants to explore the world before she settles down. One day, a strange man named Jay Woods visits her village, and Elisa is fascinated by him because he's very different. He's from America Interested about his homeland, a curious Elisa asks her father before she agrees to marry if she can visit America. She experiences cultural differences but enjoys her time in America.
ELISA
Arcler Education Inc
2017
sidottu
ELISA (enzyme-linked immunosorbent assay) revolutionized the way to detect antibodies and antigens, by using an enzymatic system instead of radioactive labelling. This technique has been widely used since its development for disease diagnostics and other purposes. A quick Pubmed search will give you more than 23000 manuscripts peer-reviewed and published, using this assay.The ELISA technique was conceptualized and developed by Peter Perlmann, and Eva Engvall at Stockholm University, Sweden. Engvall and Perlmann published their first paper on ELISA in 1971. Solid-phase techniques were used in the development of microtiter plates (96 wells) in which either an antigen or an antibody is non-covalently bound to a solid-phase support. Technical advances led to automated pipetting devices (Micromedics; Hamilton), multichannel pipettes (Lab Systems), and microtiter plate readers and washers, and in the 1980s fully automated test instruments were manufactured by Boehringer-Mannheim and Abbott, among others. Such automated systems have come to stay in medical laboratories.This book starts by introducing the ELISA history and the three different main forms of the assay, Direct, Indirect, and Sandwich ELISA. It continues with a general overview of different ELISA methods using LPS, peptides as antigens and urine the sample for diagnosis (Section 1). The next chapters focus on different forms of the ELISA assays, peptide ELISA (Section 2), microarrays, microfluidics CD-ELISA and live–cell ELISA (Section 3). The next Sections are dedicated to the application of ELISA to the diagnosis of Malaria (Sections 4), other infectious diseases (Human papillomavirus, Dengue, Prions, Cystic Fibrosis, Leptospirosis, Herpes simplex related to HIV and Hepatitis C related to HIV (Section 5), different types of Cancer (Section 6) and Alzheimer’s disease looking at biomarkers in sera and Cerebrospinal fluid (Section 7).This book is only a short overview of the big field that is the ELISA technique world. With new methods being developed daily. New methods combining this method with RFLP, PCR, microarray, microfluidics, live-cell imaging, and so on.New Infectious diseases, neurologic diseases, cancer and auto immune diseases diagnostic methods based on ELISA techniques are being commercialized and made available daily.
uana Restrepo D az(Ibagu , 1987) Periodista y Comunicadora Social de la Pontificia Universidad Javeriana. Es m ster en Escritura Creativa de la Universidad Complutense de Madrid y m ster en Estudios Literarios de la misma universidad. Ha trabajado como: jefe de redacci n de la revista Bac nika, editora de Tendencias del portal digital Kienyke.com y editora Web de Radio Nacional de Colombia. Textos suyos han aparecido en otros medios de comunicaci n como El Tiempo, El Espectador, Revista Bienestar y Revista Avianca. Actualmente, trabaja como correctora de estilo, dicta talleres de escritura creativa y escribe su segundo libro. Elisa es su primera novela.
ELISA, LE SECOND TOME DE LA S RIE ALPHA Sois mienne. C'est tout ce que je te demande. Elisa...La petite soeur d'Ethan est veuve avec deux enfants. Sa beaut et son charme exceptionnels ont aussit t conquis Jared. Mais malgr les trois ans coul s depuis le d c s de son mari, Elisa peine laisser Jared entrer dans sa vie. S'il elle est irr m diablement attir e par lui, elle ne parvient pas se d charger de la culpabilit qui la ronge. Apr s l'avoir longtemps repouss , elle se r sout finalement lui laisser une chance et s'en laisser une galement: celle de conna tre une nouvelle fois l'amour. Traumatis e par la mort violente de son poux, elle trouve aupr s de Jared la s curit dont elle a si d sesp r ment besoin. Sa possessivit et ses instincts protecteurs l'aideront-ils se sentir enti re, s' panouir et enfin tourner la page ?Jared...Jared n'est jamais tomb amoureux. Se laissant porter par le flot de la vie, il est livr au hasard des rencontres. S'il a d j t en couple, fr quent plusieurs fois la m me personne, il multiplie surtout les aventures sans lendemain. Lorsqu'il aper oit Elisa, la plus belle femme du monde, son coeur s'emballe. Ce n'est pas seulement son physique fascinant qui l'obs de, mais son coeur en or, son tre tout entier. Au premier regard, c'est le coup de foudre. N'ayant jamais rien ressenti de tel, il n'a d'autre choix que de la conqu rir. Chaque conversation, chaque contact attise la flamme de son amour; Jared adore m me ses enfants. Mais les sentiments d'Elisa seront-ils r ciproques ? Tout en sachant qu'il ne devra se contenter que d'une parcelle de son coeur, il la prot ge, la ch rit, loigne ses pires cauchemars. Mais lui brisera-t-elle le coeur ? L'in branlable amour de Jared tiendra-t-il le coup ? Ou sera-t-il an anti par la belle Elisa ?
Elisa uma jornada emocionante que atravessa alegrias e trag dias na vida de sua protagonista. A hist ria come a com um momento de decep o, onde Ben
Elisa
EifelHouse Books
2025
nidottu
