Alice Longobardi Givan

A valuable resource for introducing flow cytometry, providing a foundation for understanding its importance in various fields The newly updated and revised third Edition of Flow Cytometry: First Principles, 3rd Edition covers flow cytometric theory and the fundamentals of instrumentation and data analysis using examples from a wide range of scientific and clinical applications. Readers will find and learn about both standard and advanced flow cytometric instrumentation, methods for analyzing flow data, and applications of the technique ranging from immunology, clinical diagnoses, DNA analysis, and cell sorting, as well as novel applications such as microbiology, aquatic biology, and molecular biology. Each chapter starts with basic knowledge and gradually expands on the subject matter, enabling readers to assess the ways that flow cytometry has been used in their respective fields and the ways that it might be applied in the future. A selected number of annotated references are given, aiming to point readers into relevant literature with an extensive glossary at the end. Written by two experts and highly illustrated, Flow Cytometry: First Principles, Third Edition includes information on: How flow cytometry is used to analyze cells How a flow cytometer can be used to separate different types of cells from each other Fluidics, electronics, and optics (including lasers, fluorochromes, mirrors, and filters) Clinical applications of flow cytometry An indispensable authoritative text on flow cytometry, the third edition of Flow Cytometry: First Principles is an essential resource for scientists, clinicians, technologists, instructors, and students.

Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: *Assumes no prior knowledge of flow cytometry *Progresses with an informal, engaging lecture style from simpleto more complex concepts *Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis *Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.