Kamal Abo-Elyousr

La pourriture des racines de la luzerne caus e par Rhizoctonia solani est consid r e comme l'une des maladies les plus importantes, qui se produit chaque ann e dans de nombreuses plantes et provoque de grandes pertes de rendement. La pr sente tude visait donc trouver la meilleure strat gie pour contr ler ou au moins r duire la gravit de la maladie. Les r sultats ont montr que les isolats fongiques ont t identifi s en utilisant les caract ristiques morphologiques des myc liums comme R. solani. L'effet de certains champignons bio-agents antagonistes pour inhiber la croissance de R. solani a t tudi en laboratoire. En g n ral, l'isolat No. 2002, suivi par 2008 et 2004, qui ont enregistr (60.5, 47.2 et 33.1%), respectivement. A cet gard, la plus forte inhibition de la croissance de R. solani a t produite par l'isolat No. 5001, suivi par 5007 et 5009, 5002, qui ont enregistr (54.56, 449.39, 46.26 et 46.26 %), respectivement. Huit souches bact riennes ont t test es in vitro contre la croissance de Rhizoctonia sp. Il a t d montr que toutes les bact ries test es inhibaient significativement la croissance de Rhizoctonia sp. in vitro.

Die Wurzelf ule der Luzerne, verursacht durch Rhizoctonia solani, gilt als eine der wichtigsten Krankheiten, die j hrlich bei vielen Pflanzen auftritt und gro e Verluste in der Ertragsproduktion verursacht. Ziel der vorliegenden Studie war es daher, die beste Strategie zur Kontrolle oder zumindest zur Reduzierung der Krankheitsschwere zu finden. Die Ergebnisse zeigten, dass die Pilzisolate anhand der morphologischen Merkmale der Myzelien als R. solani identifiziert werden konnten. Die Wirkung bestimmter antagonistischer Bio-Agenzien, die das Wachstum von R. solani hemmen, wurde im Labor untersucht. Im Allgemeinen wurde das Isolat Nr. 2002, gefolgt von 2008 und 2004, die jeweils 60,5, 47,2 und 33,1 % aufwiesen. In dieser Hinsicht wurde die h chste Hemmung des Wachstums von R. solani durch das Isolat Nr. 5001 erreicht, gefolgt von 5007 und 5009, 5002, die jeweils 54,56, 449,39, 46,26 und 46,26 % aufwiesen. Acht Bakterienst mme wurden gegen das Wachstum von Rhizoctonia sp. in vitro getestet. Es wurde gezeigt, dass alle getesteten Bakterien das Wachstum von Rhizoctonia sp. in vitro signifikant hemmen.

A podrid o radicular da alfafa causada pela Rizoctonia solani considerada uma das doen as mais importantes, que ocorre anualmente em muitas plantas e causa grandes perdas na produ o de rendimento. Assim, o presente estudo teve como objectivo encontrar a melhor estrat gia para controlar ou pelo menos reduzir a gravidade da doen a. Os resultados mostraram que os isolados f ngicos foram identificados usando as caracter sticas morfol gicas da mic lia como R. solani. O efeito de certos fungos bio-agentes antag nicos para inibir o crescimento de R. solani tem sido estudado em laborat rio. Em geral isolado No. 2002, seguido por 2008 e 2004, que registraram (60,5, 47,2 e 33,1%), respectivamente Neste aspecto, a maior inibi o do crescimento de R. solani ocorreu pelo isolado No. 5001, seguido por 5007 e 5009, 5002, que registraram (54,56, 449,39, 46,26 e 46,26 %), respectivamente. Oito estirpes bacterianas foram testadas contra o crescimento de Rhizoctonia sp foi investigada in vitro. Demonstrou-se que todas as bact rias testadas inibiram significativamente o crescimento de Rhizoctonia sp in vitro.

Zgnilizna korzeni lucerny powodowana przez Rhizoctonia solani jest uważana za jedną z najważniejszych chor b, kt ra corocznie występuje na wielu roślinach i powoduje duże straty w produkcji plonu. Dlatego celem badań bylo znalezienie najlepszej strategii zwalczania lub przynajmniej zmniejszenia nasilenia choroby. Wyniki badań wykazaly, że izolaty grzyb w zostaly zidentyfikowane na podstawie cech morfologicznych grzybni jako R. solani. W warunkach laboratoryjnych badano wplyw niekt rych antagonistycznych biogen w grzybowych na zahamowanie wzrostu R. solani. Og lnie rzecz biorąc, izolat nr 2002, a następnie 2008 i 2004, kt re odnotowaly (60,5, 47,2 i 33,1%), odpowiednio. W tym zakresie, najwyższe zahamowanie wzrostu R. solani wystąpilo przez izolat nr 5001, a następnie 5007 i 5009, 5002, kt re odnotowaly (54,56, 449,39, 46,26 i 46,26 %), odpowiednio. W badaniach in vitro testowano osiem szczep w bakterii przeciwko wzrostowi Rhizoctonia sp. Wykazano, że wszystkie testowane bakterie istotnie hamowaly wzrost Rhizoctonia sp in vitro.

Il marciume radicale dell'erba medica causato da Rhizoctonia solani considerato una delle malattie pi importanti, che si verifica annualmente in molte piante e causa grandi perdite nella produzione del raccolto. Quindi il presente studio mirava a trovare la migliore strategia per controllare o almeno ridurre la gravit della malattia. I risultati hanno mostrato che gli isolati fungini sono stati identificati utilizzando le caratteristiche morfologiche dei miceli come R. solani. L'effetto di alcuni funghi antagonisti bio-agenti per inibire la crescita di R. solani stato studiato in laboratorio. In generale l'isolato No. 2002, seguito da 2008 e 2004, che ha registrato (60.5, 47.2e 33.1%), rispettivamente A questo proposito, la pi alta inibizione della crescita di R. solani si verificato da isolato No.5001, seguito da 5007 e 5009, 5002, che ha registrato (54.56, 449.39, 46.26 e 46.26 %), rispettivamente. Otto ceppi batterici sono stati testati contro la crescita di Rhizoctonia sp stato studiato in vitro .ha dimostrato che tutti i batteri testati significativamente inibito la crescita di Rhizoctonia sp in vitro.

Root rot of alfalfa caused by Rhizoctonia solani is considered one of the most important diseases, which annually occurred in many plants and causes great losses in yield production. So the present study aimed to find the best strategy to control or at least reduce disease severity. The Results showed that the fungal isolates were identified by using the morphological features of mycelia as R. solani. Effect of certain antagonistic bio-agents fungi to inhibit the growth of R. solani has been studied under laboratory. In general isolate No. 2002, followed by 2008 and 2004, which recorded (60.5, 47.2and 33.1%), respectively In this respect, the highest inhibition of R. solani growth was occurred by isolate No.5001, followed by 5007 and 5009, 5002, which recorded (54.56, 449.39, 46.26 and 46.26 %), respectively. Eight bacterial strains were tested against growth of Rhizoctonia sp was investigated in vitro .demonstrated that all tested bacteria significantly inhibited growth of Rhizoctonia sp in vitro.

Pectobacterium carotovorum subsp. carotovorum is considered one of the most important and wide spread bacterial pathogen that is responsible for great and destructive losses to the production of potato worldwide leading to the loss of hundreds of tons in the fields, transit or even during the shipment. The present study mainly carried out to assess the antibacterial activity of three essential oils against P. carotovorum subsp. carotovorum as the bacterial causal pathogen of potato tuber soft rot disease. This assessment achieved within in vitro and in vivo studies to make complete suppression of soft rot disease. Tested oils showed inhibition of the bacteria growth and in vitro experiment the best oils caused inhibition of the growth is thyme oil, colve and cumin according these results we used this oils to control the disease on potato tuber. In conclusion we could recommended that use of thyme oil at concentration 10 mg to protect potato tuber from infected of pathogen under storage conditions.

Several fungal, bacterial and viral diseases attack this crop at all stage of growth which affect of the quality and quantity of seed production. Root rot and damping-off disease caused by Rhizoctonia solani are considered to be the most important diseases, that affect seed germination and seedling emergence causing great losses in the yields. R. solani reduced rates of hypocotyl elongation in some soybean cultivars and consequently delayed seedling emergence.